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cd45 1 a20 pe cy7 thermo fisher ab 469629  (Thermo Fisher)


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    Thermo Fisher cd45 1 a20 pe cy7 thermo fisher ab 469629
    Cd45 1 A20 Pe Cy7 Thermo Fisher Ab 469629, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd45%2E1+pe-cy7+a20/Sorvall+MTX+150/pmc10499021__Supplemental_Materials-254-81-84
    Average 96 stars, based on 1 article reviews
    cd45 1 a20 pe cy7 thermo fisher ab 469629 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Staining:

    Article Title: Inflammatory signals are sufficient to elicit TOX expression in mouse and human CD8 + T cells
    Article Snippet: Reagent Fluor Clone Vendor Dilution Viability stain (1x PBS diluent, 20 min, on ice) Zombie NIR fixable viability kit (NIRViD) APC-Cy7 NA BioLegend 1:500 Surface stain (FACSWash diluent, 30 min, on ice) Fc block (CD16/CD32) Unconjugated 2.4G2 BD 1:200 CD4 PerCP-Cy5.5 RM4-4 BioLegend 1:200 CD8α AF700 53-6.7 Thermo Fisher 1:200 CD45.1 PE-Cy7 A20 Thermo Fisher 1:200 CD44 BV421 IM7 BioLegend 1:200 PD-1 BV605 29F.1A12 BioLegend 1:100 CD62L BV785 MEL-14 BioLegend 1:200 Fix (1x eBioscience FOXP3 fixation buffer, 20 min, on ice) Intracellular stain (1x eBioscience FOP3 permeabilizaiton buffer diluent, 30 min, on ice) Fc block (CD16/32) Unconjugated 2.4G2 BD 1:200 TOX e660 TXRX10 Thermo Fisher 1:200 TCF1/7 PE S33966 BD 1:200 Supplemental Table 1.

    Blocking Assay:

    Article Title: Inflammatory signals are sufficient to elicit TOX expression in mouse and human CD8 + T cells
    Article Snippet: Reagent Fluor Clone Vendor Dilution Viability stain (1x PBS diluent, 20 min, on ice) Zombie NIR fixable viability kit (NIRViD) APC-Cy7 NA BioLegend 1:500 Surface stain (FACSWash diluent, 30 min, on ice) Fc block (CD16/CD32) Unconjugated 2.4G2 BD 1:200 CD4 PerCP-Cy5.5 RM4-4 BioLegend 1:200 CD8α AF700 53-6.7 Thermo Fisher 1:200 CD45.1 PE-Cy7 A20 Thermo Fisher 1:200 CD44 BV421 IM7 BioLegend 1:200 PD-1 BV605 29F.1A12 BioLegend 1:100 CD62L BV785 MEL-14 BioLegend 1:200 Fix (1x eBioscience FOXP3 fixation buffer, 20 min, on ice) Intracellular stain (1x eBioscience FOP3 permeabilizaiton buffer diluent, 30 min, on ice) Fc block (CD16/32) Unconjugated 2.4G2 BD 1:200 TOX e660 TXRX10 Thermo Fisher 1:200 TCF1/7 PE S33966 BD 1:200 Supplemental Table 1.



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    Image Search Results


    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: G2 arrest primes hematopoietic stem cells for megakaryopoiesis

    doi: 10.1016/j.celrep.2024.114388

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: PE-Cy7 CD45.1 (A20) , Tonbo , Cat# 60-0453-U100; RRID: AB_2621850.

    Techniques: Recombinant, Adhesive, Purification, Blocking Assay, Amplification, Random Hexamer, Reverse Transcription, SYBR Green Assay, Sterility, Imaging, Software

    Journal: Cell reports

    Article Title: Kruppel-like factor 2+ CD4 T cells avert microbiota-induced intestinal inflammation

    doi: 10.1016/j.celrep.2023.113323

    Figure Lengend Snippet:

    Article Snippet: PE-Cy7 anti-mouse CD45.1 (clone: A20) , eBioscience , Cat# 25-0453-82; RRID:AB_469629.

    Techniques: Virus, Derivative Assay, Recombinant, Cell Stimulation, Enzyme-linked Immunosorbent Assay, Staining, Software

    Single-cell cloning of gene-edited functional HSCs (A) Schematic showing the extracellular domain of CD45 with allele-specific antibody clones 104 and A20 and the epitope-defining amino acid. (B) Experimental setup of the single-cell editing and expansion experiment. (C) Left: fractions of CD201 + CD150 + KSL cells in single-cell-derived cultures 14 days after cloning (n = 261 clones). Right: Histogram of CD201 + CD150 + KSL cell frequency. Zoomed-in region shows clones with >10% CD201 + CD150 + KSL cells. (D and E) CD45.1 + donor PB chimerism (D) and lineage distribution (E ) in single recipients with long-term (LT) engraftment ≥5% and multilineage reconstitution (n = 8). Numbers over graphs in (D) represent percentage of CD201 + CD150 + KSL cells in the transplanted clone (%). (F) Linear correlation plots of CD201 + CD150 + KSL cell frequency and 16-week donor chimerism. Red dots indicate LT repopulating and multilineage clones. Pearson correlation. (G) CD45.1 + PB chimerism and lineage distribution in secondary recipients (n = 5). See also <xref ref-type=Figure S4 and Table S3 . Error bars represent SD. " width="100%" height="100%">

    Journal: Cell Stem Cell

    Article Title: Controlling genetic heterogeneity in gene-edited hematopoietic stem cells by single-cell expansion

    doi: 10.1016/j.stem.2023.06.002

    Figure Lengend Snippet: Single-cell cloning of gene-edited functional HSCs (A) Schematic showing the extracellular domain of CD45 with allele-specific antibody clones 104 and A20 and the epitope-defining amino acid. (B) Experimental setup of the single-cell editing and expansion experiment. (C) Left: fractions of CD201 + CD150 + KSL cells in single-cell-derived cultures 14 days after cloning (n = 261 clones). Right: Histogram of CD201 + CD150 + KSL cell frequency. Zoomed-in region shows clones with >10% CD201 + CD150 + KSL cells. (D and E) CD45.1 + donor PB chimerism (D) and lineage distribution (E ) in single recipients with long-term (LT) engraftment ≥5% and multilineage reconstitution (n = 8). Numbers over graphs in (D) represent percentage of CD201 + CD150 + KSL cells in the transplanted clone (%). (F) Linear correlation plots of CD201 + CD150 + KSL cell frequency and 16-week donor chimerism. Red dots indicate LT repopulating and multilineage clones. Pearson correlation. (G) CD45.1 + PB chimerism and lineage distribution in secondary recipients (n = 5). See also Figure S4 and Table S3 . Error bars represent SD.

    Article Snippet: anti-mouse CD45.1-PE/Cy7 (A20) , Tombo Biosciences , Cat#60-0453; RRID: AB_2621850.

    Techniques: Cloning, Functional Assay, Clone Assay, Derivative Assay

    Journal: Cell Stem Cell

    Article Title: Controlling genetic heterogeneity in gene-edited hematopoietic stem cells by single-cell expansion

    doi: 10.1016/j.stem.2023.06.002

    Figure Lengend Snippet:

    Article Snippet: anti-mouse CD45.1-PE/Cy7 (A20) , Tombo Biosciences , Cat#60-0453; RRID: AB_2621850.

    Techniques: Recombinant, Sequencing, Software, CRISPR